Cannabis sativa hemp seed oil
The plant: Cannabis sativa is a medicinal crop that has been harvested for thousands of years. Further, it grows all over the world. For centuries, it has been useful in making clothing, paper, cordage, textiles, and more. It is quite the buzz today, because research has shown it could help with a large variety of health issues.
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Conclusion: Careful consideration of the extraction method is very important for the measurement of cannabinoids in hemp seeds. Cannabis spp. Hemp is a variety of the Cannabis sativa plant species that is grown specifically for the industrial uses of its derived products.
In most nutritional food stores and grocery stores, hemp seeds are a staple nowadays, in countries where it is legal. Hemp seeds produce negligible, if any, quantities of THC endogenously. We were interested in investigating various chemical procedures that one could employ to extract natural products, effect of solvents in these extraction methods, and ultimately the estimation of various compounds in the extract.
Three brands brand 1, 2 and 3 of hemp seeds were purchased from local supermarkets in Toronto, Canada, and were used as such in the laboratory experiments. All experiments, including extractions and analyses, were conducted under the appropriate Controlled Drugs Substances Dealer License granted to University Health Network. Four extraction methods were used to extract resins from three brands of food-grade hemp seeds.
Each brand of hemp seeds was subjected to each extraction procedure thrice to assess any variability that might arise from the extraction procedure itself. Yields of resin obtained are based on the reweighed seeds.
Microwave extraction. The sonication was repeated two additional times, collecting the solvent by decantation, refilling with an equivalent amount of solvent, and a min break between each sonication session.
Soxhlet extraction. Supercritical fluid extraction SFE. The extraction was performed using supercritical CO 2 as solvent A and ethanol as solvent B. Extracts in the form of concentrated resins were used as such for the analysis and quantification of cannabinoids. Each sample was analyzed in triplicate.
Signals on the chromatograms at retention times of 2. In addition, AUC of the internal standard was obtained from the signal at 0. These standard curves were used to estimate the concentrations of the above analytes in the extracts. Commercial hemp seeds are marketed for their high nutritional values, but due to their relationship to Cannabis spp. Hemp seeds from three brands in local supermarkets were purchased and brought to the laboratory.
Each brand of hemp seeds was subjected to four different extraction protocols, and each protocol was repeated thrice to account for any variability due to the extraction procedures and associated errors. As it is common in the Cannabis spp. THCA, tetrahydrocannabinolic acid. Extraction methods employed in this investigation utilize somewhat different principles to extract the phytocannabinoids from the hemp seeds into the solvent.
This extraction process is also expected to offer high solubility to the phytocannabinoids due to heating to higher temperatures. Sonication was conducted at an ambient temperature using ethanol as the solvent, and is expected to help release compounds from the plant materials. Among these four methods, one would anticipate the highest yield of phytocannabinoids from Soxhlet extraction. Since ethanol was used in all these extraction methods, differences in extracted quantities of phytocannabinoids can be attributed to the extraction methods themselves.
We observed large standard deviations associated with each extraction of the same brand of seeds. Each extraction was performed thrice to be able to assess the experimental variability during extraction, and based on this large standard deviation, it appears that the extracts could show reasonable variability in the assessed phytocannabinoids, and this deviation may also be due to the nonhomogenous hemp seed bulk material.
Either way, these variations warrant the analysis of multiple samples of hemp seed from different parts of the bulk material to assess total amount of phytocannabinoids, as accurately as possible. Total CBD is expected to be relatively higher in concentration in hemp seeds and is reflected in these measurements. It is interesting to note that a different brand led to a completely different profile in the phytocannabinoid variations brand 1 vs. For brand 3, three extraction methods concurred with the estimation of the phytocannabinoids, viz.
It is also noted that the phytocannabinoid content exhibited a significant variation even among batches from the same brand, reflecting both the inhomogeneous nature of seeds as well as the variations in quantification based on the extraction process. Using a similar hexane-sonication procedure, quantification conducted by Ross et al. This inconsistency could be attributed to the higher oil content within hemp seeds compared to the rest of plant. Chinello et al.
In an earlier study, Ross et al. In the report by Chinello et al. This suggests the importance of heating and prolonged solvent cycling in extracting phytocannabinoids from lipid-rich materials such as hemp seeds. A more robust extraction methodology such as Soxhlet extraction may be more appropriate for the testing of hemp seed products.
One may also consider employing washing of hemp seeds with ethanol or other similar solvents, to remove any contamination to the seeds before packaging; but such change from current practice and new processes must be thoroughly investigated before implementation for consumer marketing.
Based on the above findings, it is also recommended that the hemp seeds be analyzed specifically for phytocannabinoid content before release into consumer markets. National Center for Biotechnology Information , U.
Journal List Cannabis Cannabinoid Res v. Cannabis Cannabinoid Res. Published online Oct 1. Yi Yang , 1,, 2 Melissa M. Lewis , 1,, 3 Angelica M. Bello , 1,, 2 Ewa Wasilewski , 1,, 3 Hance A. Clarke , 4,, 5 and Lakshmi P. Find articles by Yi Yang.
Melissa M. Find articles by Melissa M. Angelica M. Find articles by Angelica M. Find articles by Ewa Wasilewski. Hance A. Find articles by Hance A. Lakshmi P. Find articles by Lakshmi P. Author information Copyright and License information Disclaimer. This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
This article has been cited by other articles in PMC. Associated Data Supplementary Materials Supplemental data. Introduction Cannabis spp. Materials and Methods Materials Three brands brand 1, 2 and 3 of hemp seeds were purchased from local supermarkets in Toronto, Canada, and were used as such in the laboratory experiments.
Extraction Four extraction methods were used to extract resins from three brands of food-grade hemp seeds. Results and Discussion Commercial hemp seeds are marketed for their high nutritional values, but due to their relationship to Cannabis spp. Open in a separate window. Table 1. Supplementary Material Supplemental data: Click here to view. Acknowledgments L. Author Disclosure Statement L.
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Hempseed oil is made from the hemp seed itself and usually doesn't contain any THC (tetrahydrocannabinol), the psychoactive component. Hemp seed oil comes from the small seeds of the Cannabis sativa plant. The seeds do not contain the same levels of compounds as the plant.
As cannabis and hemp become more extensively researched and widely available, we are starting to realize the true therapeutic power of these plants and their derivatives. Today, we're clearing up the murkiness surrounding cannabis products, their differences, and the legality of taking them so we can focus on all the benefits of hemp oil extract as well as the ins and outs of buying and consuming it. There are many varietals of the Cannabis sativa plant , all of which contain different amounts of THC the psychoactive component , CBD a nonpsychoactive component , and other components.
Hemp oil comes from the Cannabis Sativa plant.
This stepping stone came in the form on a farm bill that was passed, and not just any farm bill. This farm bill was monumental because it legalized the production of industrial hemp throughout the US.
Hempseed oil vs. CBD oil
Hemp oil, more accurately called hemp seed oil, is used in many topical skin care products and cosmetics. Although as consumers we shorthand it to "hemp oil," hemp seed oil is the cannabis product most often used in over-the-counter skin care products and cosmeceuticals. What is unique about hemp oil and why are so many people using it in their skincare routines? Hemp seed oil is obtained from pressing the seeds of the cannabis plant Cannabis sativa L. Unrefined hemp seed oil is a dark greenish color with a mildly nutty aroma.
Cannabis Sativa Seed Oil
Hemp oil hemp seed oil is oil obtained by pressing hemp seeds. Cold pressed, unrefined hemp oil is dark to clear light green in color, with a nutty flavor. The darker the color, the grassier the flavour. It should not be confused with hash oil , a tetrahydrocannabinol -containing oil made from the Cannabis flower. Refined hemp seed oil is clear and colorless, with little flavor. It is primarily used in body care products. Industrial hemp seed oil is used in lubricants, paints, inks, fuel, and plastics. Hemp seed oil is used in the production of soaps, shampoos and detergents.
Conclusion: Careful consideration of the extraction method is very important for the measurement of cannabinoids in hemp seeds.
When trying to wrap your head around the differences between hemp and cannabis, it is important to begin with this simple concept: Both hemp and cannabis ultimately come from the same plant… just different parts. Whether you call something hemp or cannabis will depend on a variety of factors which we will explore in this article.
Cannabis sativa: Hemp Seed Oil vs CBD for the Skin
Organic Hemp Seed Oil. Hemp seed oil has one of the highest antioxidant levels of any vegetable oil. Phytosrerols, phospolipds as well as a number of minerals including calcium, magnesium, sulfur, potassium and phosphorus are also found in hemp seed oil. Many Formulators are adding hemp seed oil to their skin care applications because it contains a high amount of essential fatty acids, which makes it an important ingredient in anti-inflammatory skin care formulations. It is also non-greasy, emollient and moisturizing compound with excellent anti-aging and moisture balancing properties. INCI Nomenclature:. Cannabis Sativa Seed Oil. Suggested Use Levels:. Green to Dark Green Oily Liquid. This product should be added to a formulation at the recommended usage rate. Find Information.
What Is Hemp? Understanding The Differences Between Hemp and Cannabis
You have! The Cannabis sativa plant is a unique herb used in everything from textiles to food to skincare. Hemp and Marijuana are from the Cannabis sativa species but are genetically different and are used for different purposes. Cultivated for use in everything from agriculture to food to skincare, Hemp is a multi-purpose ingredient with a range of benefits. Hemp is a well-known fiber used in clothing, textiles, and paper.
